Prostate Specific Antigen (PSA) Semen Detection
- The Prostate Specific Antigen (PSA) Test Cartridge is based
upon the following principals.
- The membrane contains two monoclonal murine anti-PSA
antibodies as active compounds. One of these antibodies is
immobilized at the test region on the membrane. The
upstream control region and the region of the internal
standard (between control and test region) contain
immobilized polyclonal goat anti-mouse antibodies. The
amount of antibody at the internal standard is adjusted to
a color intensity of the line, which is equal to the color
intensity of the test line at a PSA concentration of 4
ng/mL. A glass fiber pad downstream of the
membrane is used for sample loading and transmission to a
second fiber pad with the dried and gold labeled second
monclonal murine anti-PSA antibody.
- PSA at the sample will bind to the re-mobilized
gold-labeled antibody and form a
PSA-gold-labeled-anti-PSA-antibody-complex. Through the
capillary effect of the membrane, the reaction mixture
including the complex is carried upwards with the fluid.
In any case the colored gold labeled anti-PSA-antibody
will bind to the anti-mouse-antibody at the control region
and the region of the internal standard thus developing
two red lines (one at the control region ant one at the
region of the internal standard). These two lines are
independent of the existence of PSA at the sample and
indicate only the correct execution of the test.
- If the sample contains PSA the PSA-gold-labeled
anti-PSA-antibody complex will bind to the immobilized
monoclonal antibody of the test region that recognizes
another epitope on the PSA molecule. The binding is
indicated by the formation of an additional line. Thus a
PSA positive sample will show three colored lines in the
result well. The line in the middle (internal standard)
correlates with an amount of 4 ng/ml PSA. In some cases it
might be helpful to estimate the amount of PSA in the
sample by comparison of the result line with the internal
- These raw cartridges or complete test kits can
be purchased in quantities at ProstateSpecificAntigen.net.
Individual test kits can be purchased directly from this
website at this link.