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Semen on Panties
The evolution of the semen detection

Semen and Sperm Detection, Inc. provides an accurate and conclusive examination for the detection of semen and sperm.  Our 4-phase test is the most accurate and reliable semen and saliva detection test  available.  order here


History of Semen Detections

ORFILA Mathieu Bonaventure
Mathieu Bonaventure Orfila (1787-1853)

Professor of medicinal and forensic chemistry at Univ. of Paris, Considered the father of modern toxicology. Credited as the first to attempt the use of a microscope in the assessment of semen stains in 1813.  Orfila published Traité des poisons tirés des règnes minéral, végétal et animal, ou Toxicologie générale Paris, chez Crochard, 1818. 

HL Bayard, "Forensics in a jury trial" published the first reliable procedures for the microscopic detection of sperm in 1839

A. Florence of Lyons develops use of reagent "teriodide of potassium" creating a crystalline reaction for semen in 1896.

Dr. W.F. Whitney of Boston MA develops the method of fixing and staining semen (currently referred to as "Christmas Tree Stain")  for microscopic inspection for spermatozoa in 1897.

Frank Lundquist, working at the Legal Medicine Unit at the University of Copenhagen, developed the acid phosphatase test for semen in 1945.  

Claude B. Hazen published Measurement of Acid Phosphatase Activity to Identify Seminal Stains  The Journal of Criminal Law, Criminology, and Police Science, Vol. 46, No. 3 pp. 408-413 in 1955.

M. Hara et al. first described a protein in the seminal fluid named gammaseminoprotein in 1971.  

Gammaseminoprotein was characterized in detail by Sensabaugh et al. who found that its molecular weight corresponds to 30,000 Dalton and thus named it p30 in 1978.

P30 was called Prostate Specific Antigen (PSA) and first purified by Wang et al. in 1979.

DNA Testing Centre Inc (DTC) offered online purchases of laboratory semen detections in 2000.

DTC developed an in-house laboratory procedure for a 3 part semen detection including Prostate Specific Antigen (PSA), microscopic inspection , and the Acid Phosphatase (AP) test in 2002.

DTC developed and sold the first non-presumptive home semen detection test in 2003.

Semen and Sperm Detection Inc (SSDI) was formed as a corporation dedicated to developing and performing semen detection tests in 2005.

In February 2007, SSDI added the saliva detection to every standard test, at no additional charge.

In August 2011, SSDI added a sperm protein (SP10) test to compliment a positive PSA test result.  SSDI is developing and validating the SpermCheck® Forensic cartridge for resale.

On 21 June 2012, SSDI became the only stand-alone ISO17025 forensic accredited laboratory in existence.

SSDI has performed 3000+ laboratory semen detections and has sold 47,000+ PSA semen detection test kits.